FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13879530
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA175300.08.5009319
AGAGCAC199200.07.625558
GATCGGA225450.06.7682131
GGGGTCC50600.06.74921
GGGGGTC53100.06.568281
CCCCCCT30452.5102054E-106.44290071
TCGGAAG247550.06.3457093
AAGAGCA251800.06.2613287
ATCGGAA268100.06.13077972
GGGGGGA38559.094947E-126.0315731
GGGGGTA42201.8189894E-115.68206831
GGGGGCT69700.05.52520041
GGGGGTT38951.4830221E-85.2234271
GGGGTCT53453.6379788E-125.16583871
GAAGAGC318500.05.1312996
Produced by FastQC (version 0.11.5)