FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13879530
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTA44500.06.22983461
GGGGTAG59350.05.77736851
GATCGGA224500.05.39441821
GGGGTCC50553.6379788E-125.33990431
AGAGCGT234550.05.3050498
GGGGTCT56800.05.26609131
TCGGAAG249550.05.21616363
GGGGCCC45156.2755134E-105.170651
ATCGGAA268050.05.1526972
AAGAGCG261700.05.13937577
GGGGGCT75500.05.024691
Produced by FastQC (version 0.11.5)