FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14708024
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA190250.013.87807751
TCGGAAG218300.012.0049883
ATCGGAA225000.011.8716982
AGAGCGT236250.010.6656348
AAGAGCG263500.010.3822267
GAAGAGC286300.09.5806636
GGAAGAG289600.09.5709625
GAGCGTC271600.09.1447939
TCATTAA60650.08.19056960-64
TAGATCT64300.08.17383740-44
CGGAAGA353650.07.87858874
CATTAAA71000.07.442751460-64
ATCATTA73300.06.44311160-64
CCTAGAT5855.5424607E-96.1536856140-144
TAGGGAA89100.06.059669515-19
GTAGATC93450.06.039737735-39
GTAGGGA92550.05.802668615-19
GTGTAGA103600.05.60088435-39
ACTGGGT97200.05.59959530-34
CTAGATC12500.05.529456140-144
Produced by FastQC (version 0.11.5)