FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14610710
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT21800.016.3597581
TAGCATG25350.015.6197628
GTGAGTA27700.013.0077062
CATTTAG28500.012.38913254
TAACCCC37450.011.9187179
ATTTAGC44000.08.508315
ATAACCC54200.08.3681788
TTAGCAT52000.07.6146347
GAGTATA59950.06.7311384
TATAACC63300.06.71023947
GACCCTA18850.06.41636585-89
TGAGTAT62150.06.1452463
TTTAGCA69450.05.9087056
GTATAAC62550.05.8699256
TCATTTA71650.05.6321783
CTAACTA18500.05.058961125-129
Produced by FastQC (version 0.11.5)