FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13867918
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA130150.015.0499271
AGAGCGT131950.014.1324368
AAGAGCG159250.012.9304377
TCGGAAG171000.012.0840463
GAGCGTC168950.011.2078989
ATCGGAA194100.010.6833172
GAAGAGC217000.09.3897346
TGTGTAG50200.08.29005335-39
TAGATCT50900.08.11950440-44
GGAAGAG255700.07.9967635
CGGAAGA306150.06.96119934
GTAGGGA64550.06.692627415-19
TGTAGGG68300.05.96674415-19
TAGGGAA74000.05.876880615-19
TCGTGTA77900.05.56398110-14
GTAGATC78750.05.48572335-39
AGATCTC83350.05.355764440-44
TTGTGTA83450.05.194016535-39
GTGTAGA88300.05.10442535-39
GTGTAGG86000.05.00661715-19
Produced by FastQC (version 0.11.5)