FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12497977
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA101000.017.0666121
GAGCACA114250.014.2680689
AGAGCAC124600.013.2002168
TCGGAAG133900.012.0998123
ATCGGAA154050.011.1247922
AAGAGCA207350.08.3953967
CCCCCTA22901.8189894E-128.2576831
CGGAAGA218100.07.89069754
GGAAGAG239250.07.6372935
GAAGAGC247100.07.1321966
CACACGT63300.05.543117510-14
CACGTCT69500.05.406234310-14
ACACGTC71600.05.04348210-14
Produced by FastQC (version 0.11.5)