FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14283021
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCCC92800.014.5064968
CCCGGCC127000.011.5734171
GTACACA71250.011.3165145
CCGGCCG127450.011.0189852
CGCGCCC124350.010.8838487
ACACAAG80850.010.2397237
TACACAA80750.09.62834556
GGCCGCG150300.09.4368014
CGCCCCG136850.09.3110239
GGTACAC87850.08.6052824
CGGCCGC181500.08.0922833
AGGTACA98650.08.0281073
AAGGTAC104550.07.7839712
GCCGCGC211350.06.8465755
CCGCGCC208450.06.6653946
CACAAGG127950.06.5265938
Produced by FastQC (version 0.11.5)