FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12825575
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACA37900.011.0175035
TACACAT37700.010.884866
ACACATA36150.010.7541177
TACGTAC40850.010.0553132
ATACGTA47200.09.0965571
CCTAGAC8750.08.721418115-119
CACATAC51400.08.2637698
ACGTACA58250.07.79379463
GAGCACA105500.07.43812279
ACGTTAA23402.8740033E-107.39068134
ACATACT60400.07.1516079
CTAGACC11350.07.1041446120-124
TACGTTA27402.3646862E-117.10096743
CGTACAC68150.07.0843174
GGTCCTA11250.06.527371440-44
TCCTAGA13300.06.4955945115-119
ATCCTAG12950.06.4487796115-119
AGAGCAC137250.05.92727958
GATCGGA165150.05.5961861
CCTAATG30153.279638E-75.5514641
Produced by FastQC (version 0.11.5)