FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12825575
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT41300.011.6782296
ACACATA36200.011.533787
TACGTAC39100.010.67974952
GTACACA40200.010.386145
ATACGTA46500.010.0290541
CACATAC51850.09.162928
CCTAGAC9300.08.979384115-119
CTAGACC11950.07.590602120-124
ACGTACA61700.07.2342923
ACATACT64700.06.67552859
TCCTAGA14400.06.3991017115-119
CGTACAC72800.06.3290444
GGTCCTA13450.06.20941440-44
ATCCTAG14800.06.031586115-119
GTCCTAA17600.05.809385345-49
CTAACAC18950.05.39416125-29
CCTAAGG18850.05.27135545-49
TCGGAAG174100.05.12757973
Produced by FastQC (version 0.11.5)