FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15136438
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA187300.011.7851681
TCGGAAG231250.010.117313
AGAGCGT220800.09.8462928
ATCGGAA249300.09.0714942
GAGCGTC254000.08.7010069
AAGAGCG267300.08.5373917
CGGAAGA320100.07.4439894
GGAAGAG316850.07.2704475
GAAGAGC325600.07.1413936
TAGATCT72450.06.360077440-44
TGTGTAG80950.05.69277935-39
CCTTCAC186800.05.59533071
CCTTGAT213750.05.16341971
CCTTCAT195850.05.1128571
Produced by FastQC (version 0.11.5)