FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005084004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005084004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18033988
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAA85850.07.27907851
CCTGATC220050.06.50523761
CCTGACG229300.06.05267861
CCTGACC287600.05.8868821
GGTATCT147900.05.79738471
CCTGAAT176950.05.74904541
GGTATAC66350.05.69482951
GGTACCA98350.05.6889831
GGTATGG149050.05.55764961
GGTAGAG183250.05.43243931
GGTTAGA25702.2066033E-55.3720341
GGTAGCA98600.05.3060811
GGTACTG129850.05.2602231
GGTAAGG147500.05.2219431
GGTAAAC142350.05.15563541
GGTAAGA144200.05.13988261
GGTACAG153300.05.11917351
GGTAGTG78300.05.10411
GGTAAAG204350.05.08486751
GATCGGA237700.05.04397631
Produced by FastQC (version 0.11.5)