FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005084274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005084274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16380734
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACCC86050.020.073218
CAACCAC98550.017.7466644
ACCACAC102100.017.270236
ACACCCT104350.016.6219149
CCAACCA113450.016.1770783
ACCAACC114750.015.6810662
AACCACA129150.013.4858635
CCACACC145400.012.2756297
CACCAAC157450.011.53961
GTCAGTC110750.08.3182754
AGTCCAT113400.07.8063597
GGGGTCC51000.07.764683235-39
CAGTCCA120650.07.5758996
TCAGTCC127700.07.15765245
GGTCCTA57250.07.11823235-39
GGGTCCT57900.07.11293235-39
GTCCATC129850.07.0945448
TGTCAGT142400.06.46940573
CTAGATC12250.06.348779140-144
GGGGGTC68300.06.19991530-34
Produced by FastQC (version 0.11.5)