FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005084652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005084652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14084727
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT42950.025.9837021
TAAGGGG47300.023.594089
GTGAGTA52050.021.9942362
TAGCATG55400.018.7148028
GTCCCTT64350.016.4475521
ATAAGGG92150.012.579498
CCCTTTA95150.011.7288493
TCCCTTT139650.08.5585392
GAGTATA144900.08.4968954
TGAGTAT142500.08.48842053
GTATAAG163200.07.3676476
CCTTTAG159300.06.9152544
AGCATGA166300.06.88394459
TATAAGG177650.06.7683657
GTGGGTC41900.06.32362815-19
ACTAACC46500.05.9148383125-129
GACCCTA44150.05.773046585-89
AGGGGTG49350.05.719148610-14
CTAACCA48700.05.5885005125-129
CTAGGAG47800.05.362343350-54
Produced by FastQC (version 0.11.5)