FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005084653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005084653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14084727
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT43550.025.460391
TAAGGGG51250.021.4946339
GTGAGTA51350.021.3125612
TAGCATG59600.016.9127528
GTCCCTT63400.016.0126191
ATAAGGG89900.012.8943278
CCCTTTA94900.010.9251843
GAGTATA146300.08.1695164
TGAGTAT143900.08.0055593
TCCCTTT141850.07.76594972
GTATAAG169200.07.23404266
CCTTTAG159950.06.977184
TATAAGG174900.06.62778767
AGCATGA173400.06.39446359
CTAGGAG45150.05.868438750-54
GTGGGTC45700.05.57724315-19
GACCCTA44100.05.485714485-89
AGGGGTG52950.05.19433410-14
TTAGCAT239950.05.13106877
ACCCTAT51350.05.01966985-89
Produced by FastQC (version 0.11.5)