FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005084743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005084743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18063640
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA13800.015.6521743
CGTCTAA22250.09.7078653
CGACTAA19302.9758667E-97.8341973
TAGGCTA24951.8189894E-127.7915832
GAGCTAA30003.092282E-116.71999933
GACTCTA25458.860479E-86.2239692
TTAGGCT33557.6397555E-116.22354751
TAGAGTA34251.3096724E-106.096352
GCTCTAA32801.8389983E-95.9268293
ACTCTAA28009.1464244E-85.91428573
CTACTAA30551.4426405E-85.89198063
AGATCTA45601.8189894E-125.6842112
GTACGTA34503.5497578E-85.4260872
CTCCTAA36851.5388287E-75.08005433
Produced by FastQC (version 0.11.5)