FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12987765
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATGTACATCTCGTAT401670.3092679918369327TruSeq Adapter, Index 2 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA176550.048.655061
GAGCACA179500.046.0011069
AGAGCAC186550.044.2626578
TCGGAAG207800.040.7295153
ATCGGAA236750.036.3100662
AAGAGCA298400.028.4918337
CGGAAGA302100.028.1667084
GGAAGAG308700.027.3079815
GAAGAGC326750.025.8435276
TTAGACC23550.017.424227
TAGACCC27150.016.4395988
ATTAGAC26850.015.8189296
AGTCACA87250.015.69365425-29
CAGTCAC101350.014.87414325-29
CACACGT121450.014.14302310-14
CCAGTCA117500.013.36890725-29
GTCACAG103000.013.22400325-29
CACGTCT128200.013.17374710-14
ACACGTC132150.013.09593810-14
TAATTAG37600.012.4449994
Produced by FastQC (version 0.11.5)