FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12987765
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGATGCTGATAGTGTAGATCTCGGT504530.3884656058990904Illumina Single End PCR Primer 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA176350.046.2780571
TCGGAAG205300.039.2743573
AGAGCGT227450.035.256368
ATCGGAA231700.035.216092
GAGCGTC243550.032.8964169
AAGAGCG260450.031.1211457
CGGAAGA303050.026.8194354
GGAAGAG313300.025.8493965
GAAGAGC330050.024.6029726
TTAGACC23600.020.7414177
ATTAGAC25550.018.8773986
TAGACCC27500.017.799778
TAGTGTA95250.017.0339935-39
TAGATCT99900.016.5155140-44
AGTGTAG103750.016.15185435-39
ATAGTGT109050.015.01034330-34
TAATTAG35400.014.03187754
AGATCTC125700.013.62964640-44
GTAGGGA122450.013.50952615-19
GTAGATC126900.013.27338635-39
Produced by FastQC (version 0.11.5)