FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16422407
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATTCATCTCGTAT393710.23973952174002264TruSeq Adapter, Index 25 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA280100.024.4661459
AGAGCAC301050.022.8115128
GATCGGA307950.022.800791
TCGGAAG340300.020.4870033
ATCGGAA374950.018.8437772
AAGAGCA391500.017.7251387
GGAAGAG471750.015.137155
GAAGAGC479600.014.6942646
CGGAAGA502500.014.2995844
AGTCACA134450.010.12050525-29
CTCGTAT129650.09.89509640-44
GTCACAC142200.09.42716925-29
TCACACA145050.09.0722730-34
CAGTCAC157900.08.97313525-29
TCGTATG154350.08.321094545-49
TCTCGTA158600.08.30677940-44
CCAGTCA176950.08.20240425-29
ACACAGA170050.08.04330430-34
CACACAG166550.08.02215230-34
ACTCCAG180500.07.961067720-24
Produced by FastQC (version 0.11.5)