FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15359677
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA171400.033.5433731
GAGCACA172150.032.618869
TCGGAAG189550.030.1206653
ATCGGAA197100.029.4050242
AGAGCAC191100.029.3846668
CCCCCAA76600.028.3483661
CCCCAAA98500.022.8781242
AAGAGCA254350.022.3039267
GGAAGAG293600.019.5183835
CCCAAAA119350.018.6402263
CGGAAGA318500.018.26374
GAAGAGC376500.015.469296
CCAAAAA158050.014.0295294
CACACGT110600.010.64740510-14
CAGTCAC76900.09.81224625-29
CCAGTCA86000.09.46048725-29
CACGTCT129150.09.17383810-14
ACTCCAG118200.09.08686120-24
TCTGAAC138450.08.90043815-19
GTCTGAA139450.08.66111915-19
Produced by FastQC (version 0.11.5)