FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15359677
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGATTAGGCATGTGTAGATCTCGGT254180.16548525076406229Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA169700.030.5676571
TCGGAAG186800.026.6343633
ATCGGAA198900.025.376072
AGAGCGT231700.020.1659568
AAGAGCG263650.018.6234267
GGAAGAG295000.016.7383145
CGGAAGA317950.015.6930394
GATTAGG63600.015.50549125-29
GAGCGTC314850.015.4119059
AGATTAG63750.015.4012625-29
ATTAGGC65100.015.23667825-29
TTAGGCA70250.014.38609425-29
TGTGTAG74850.013.67513435-39
GAAGAGC378150.013.3652156
TAGATCT88900.011.49802840-44
TAGGGAA90950.011.33324215-19
CATGTGT94650.010.98184130-34
GTAGGGA98950.010.63519515-19
ATGTGTA97600.010.47278835-39
TCATTAA90900.010.10676360-64
Produced by FastQC (version 0.11.5)