FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16074379
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTA41550.023.400072
AGTGAGT42000.022.2935851
TAGCATG54100.016.8976298
TAACCCC62350.016.047149
CCACTCA49500.013.8145819
ATAACCC97450.010.341078
GCCACTC68250.010.2303018
GTATAAC116600.09.3853866
TGAGTAT121100.08.7988253
CCTTTAG104800.08.5185864
TATAACC125150.08.4565577
GATCGGA176900.08.3943561
AGCCACT91300.08.3587677
CTAGGAG26300.07.99500250-54
AAGCCAC105050.07.95003466
GAGTATA130900.07.81008634
AGAGCGT191850.07.80406438
TCGGAAG202350.07.40060573
AAGAGCG217800.07.00785547
ATCGGAA226650.06.8318322
Produced by FastQC (version 0.11.5)