FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11739558
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCAA55950.018.2412551
CCCCAAA82250.012.6057612
CCCAAAA101850.010.3213853
GAGCACA83300.08.6418899
CCAAAAA137100.08.1386364
AGAGCAC98750.07.58147348
GATCGGA120200.06.97461271
AAGAGCA144250.05.93866067
TCGGAAG139350.05.52869943
ATCGGAA144150.05.44445372
CCCCCTA21853.499708E-45.3382061
CAAAAAA232450.05.3266765
Produced by FastQC (version 0.11.5)