FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16094693
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT44850.035.5956271
ACGTTAA45050.035.0780074
TACGTTA46650.034.6269533
CCACTCA51850.023.5191829
CGTTAAA68800.023.0738375
TTACGTT82500.019.9026052
GATCGGA192550.017.9767531
GCCACTC75350.016.7587038
TCGGAAG213200.016.5739783
GTTAAAT108600.015.0827886
ATCGGAA243150.014.601642
AGAGCGT232800.014.0720868
AAGAGCG259950.013.0742487
AAGCCAC102100.012.8626746
AGCCACT101800.012.3335217
GAGCGTC267250.012.1500789
TAACCCC39600.011.4796769
GGAAGAG310550.011.1298575
TACAAAG127050.011.0532232
GGCCTAA27200.011.01061975-79
Produced by FastQC (version 0.11.5)