FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12934789
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT33350.011.5760811
GTGAGTA35400.010.7929682
TAGCCCC38650.09.310329
CCACTCA32500.08.1933699
GTATAGC71800.06.31502876
GCCACTC46450.06.1975988
TAGCCAC37002.3646862E-116.03037554
CCCTAAG22101.3326322E-55.8619146
TATAGCC80150.05.2080927
CACTCAA49256.730261E-115.11459168
AGTATAG82050.05.08768655
ATAGCCC81250.05.0489458
Produced by FastQC (version 0.11.5)