FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16048507
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA35300.012.2352099
GCCACTC61600.07.94626478
AGCCACT78700.06.31116067
ATCTAGC14450.06.0781235-39
CCGTTAA94900.05.61331374
CTAGCTC15800.05.376622740-44
CGTTAAA102050.05.3611065
GTTAAAT97650.05.30787666
AAGCCAC98750.05.17585136
TCCGTTA105800.05.1030133
CATCTAG17400.05.047634635-39
Produced by FastQC (version 0.11.5)