FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18100281
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA186600.014.8553069
AGAGCAC195150.014.0937758
GATCGGA215600.013.2578851
TCGGAAG267400.010.6088263
AAGAGCA282500.010.1946917
ATCGGAA301600.09.6445622
GAAGAGC347550.08.4730256
GGAAGAG373700.07.9571855
CGGAAGA416300.07.19481134
AGTCACG88050.06.50902925-29
CTCGTAT96700.06.12037340-44
CAGTCAC100700.05.877259725-29
CTAGATC14250.05.5578947140-144
CCAGTCA115150.05.202257625-29
GTCACGT112950.05.201593425-29
TCTCGTA114050.05.176676840-44
CACACGT116250.05.053935510-14
Produced by FastQC (version 0.11.5)