FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13776929
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAAC23750.048.208351
CTAACGT26800.042.9794773
TCTAACG28850.039.9261972
AGGTTAG29600.035.994929
TAACGTG42450.027.3038644
ACGTGAG55650.021.7327826
CGTGAGT53200.021.3804427
CAGGTTA56500.019.4945938
ATCGTTA72200.017.4844721
TAGCCTC75100.017.2545558
GTGAGTT70500.016.7465728
ATCGGAC94700.014.7253271
GGACGTT91950.014.7191274
ACGTTGG91100.014.4611856
TACAGGT79350.014.0622886
GTTATCC94550.013.6291084
AATAGCC93400.013.56553656
AAATAGC90850.013.5502435
TCGTTAT93050.013.4622192
CCACAGA100250.013.0694769
Produced by FastQC (version 0.11.5)