FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15921766
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTCC130350.014.7452635
AAGGCTC128250.014.5937974
CTCCAGA140150.013.1491968
ACCCCCC50550.012.6742189
TCCAGAC153400.012.1542099
GGCTCCA158650.011.9788796
AGAAAGG193550.010.5725481
AAAGGCT187100.010.3904673
TGAGTAC67350.010.2743992
GAAAGGC203900.09.6155572
AGTACAC72550.09.128574
GCTCCAG230200.08.3807297
ATGAGTA84150.08.3099331
GAGTACA96150.07.56340173
TACACCC96050.07.41976556
ACACCCC123850.06.5680247
GTACACC119700.06.25448475
GTGACTG71850.05.73094155-59
GGTGACT84700.05.065467455-59
CACCCCC172450.05.0092238
Produced by FastQC (version 0.11.5)