FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12013998
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGT10850.011.280422115-119
ACTAGTG14500.09.235164100-104
CTAGTAC14400.09.199442115-119
TGACTAG13000.09.193289115-119
CTAGTTG14050.08.916184110-114
CACTAGT15800.08.475329105-109
GTATACA72250.08.1107551
CTAGTGC16450.08.052887100-104
GCACTAG18200.07.120359105-109
GGTCCTA19401.9503932E-77.0854261
AACTAGT20800.07.0610046100-104
ACTAGTT20150.07.002962105-109
GTGTATG76150.06.93532851
TCGCTCT100500.06.87740669
GTATATT98600.06.82370331
AAACTAG21000.06.71949295-99
TTCGCTC104450.06.4104788
AATTTCG108100.06.3270495
CAAATTT114450.06.2971383
GTATAGG58150.06.22062971
Produced by FastQC (version 0.11.5)