FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14102628
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACTA26850.017.6919657
ACACACT41900.011.8524576
ACACTAT44950.011.368638
CCTAGTA6900.010.85133785-89
CACTATA46700.010.6345589
AACACAC51200.010.4032125
CCCTAGT7300.010.05949985-89
CAACACA58600.09.4598014
CCTAGAC14150.08.751809120-124
ATCCTAG20900.07.716354115-119
CTAGACC22100.07.2976255120-124
ACCCTAG10550.06.8239480-84
ACAACAC94250.06.64468433
ATACAAC99500.06.279271
CTAACAC29600.06.17684625-29
TACGTAC57600.06.000272
CTAGTAC14900.05.89484885-89
GTCCTAA32850.05.565722545-49
CGTTAAA50601.8189894E-125.40553575
CCTAAGG33350.05.395943645-49
Produced by FastQC (version 0.11.5)