FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18012466
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAC51100.014.6522147
CCTCACA60650.012.1075936
CCCCTAG11950.010.84399115-19
ACCCAAC63650.010.0665915
ACCCTCA81950.09.4928084
CCCTAGA14650.09.14028815-19
CACACCT87350.08.9013889
CCTAGAC16350.08.630239515-19
TCACACC95200.08.6210218
CCCTCAC91750.08.5528585
GCACCCT111950.07.78559972
CACCCAA88800.07.3004484
CTAGACT19750.07.290298520-24
CCCAACC90050.07.1952726
AGCACCC143750.06.815141
CACCCTC124750.06.81353143
CCACCCA115300.06.06000853
CCAACCT108550.05.96902667
AACCTCA113950.05.8125999
Produced by FastQC (version 0.11.5)