FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17210055
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTGT48300.035.6270649
GGTAGTG90800.020.0615238
ACGGTAG107400.016.8937786
ATCCGAC116100.016.3140771
CGGTAGT114100.016.0910747
GGACTAG12950.012.78754215-19
TCCGACG176350.011.0648572
ACTAGCC14700.010.87345820-24
CAAGTTG73700.010.8439127
GACTAGC14150.010.685520-24
GACGGTA183750.010.3836265
CTAGCCT15900.010.14338520-24
CTAGCTC15650.09.66129245-49
CACTCTA27400.08.9364991
CGACGGT244650.08.0637274
TCAAGTT106550.07.70339066
TCTAGCT22100.07.6234945-49
TTCAAGT116750.07.4003945
AGTTGAT118850.07.3301939
AAGTTGA124000.06.85156158
Produced by FastQC (version 0.11.5)