FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15580062
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTGT46900.029.4754189
GGTAGTG76800.019.124968
ACGGTAG93550.015.7776246
GGACTAG10800.015.33325115-19
GACTAGC10900.015.06051720-24
CGGTAGT105000.014.0571137
CTAGCCT11050.013.94386220-24
CTAGCTC11100.013.88106945-49
ACTAGCC11450.013.83403120-24
ATCCGAC114450.013.3401381
TCTAGCT12850.012.66302345-49
CAAGTTG70550.012.1445537
TTCTAGC14800.010.60539545-49
GACGGTA156800.010.1020185
TTTCTAG17250.09.76694840-44
TCAAGTT95350.09.6654226
AAGTTGA103050.08.7336058
AGTTGAT103300.08.573079
TTCAAGT100050.08.56375
TCCGACG189400.08.4015992
Produced by FastQC (version 0.11.5)