FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005085987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005085987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12425595
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAT70850.012.6760411
GTACAAG111600.012.3307981
GTCTATA79050.012.2773511
GTGCAAC76100.011.8015431
GTGTAAG52750.011.6707361
GTGTTCA112950.011.1574461
GTGCAAT81900.010.4351791
GTACAAC90800.010.3695351
GTGTAAC36000.010.0593361
GTATAAG85000.09.5433511
GTGTACG72100.09.442661
GTGCACT39650.09.3159861
GTATAAC54900.08.9709481
GTGCAAA126850.08.9070921
GTGTAAA91050.08.8296771
GTGTAAT56300.08.6192251
GTATACG54800.08.5908191
TATGACC91400.08.3491936
GTGTAGC75050.08.2994541
GTGCAAG85650.08.1179361
Produced by FastQC (version 0.11.5)