FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14527016
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGT46950.07.9695551
GTATAGG56600.07.7773971
GTATACT39800.07.5578841
GTATAGA64550.06.93318941
GTATACG50800.06.7878721
GTATGGA88500.06.71492621
GTATATC105200.06.69506741
GTATATG87350.06.55135541
GTATGCG106150.06.49691721
GATCGGA129550.06.3994191
GTATAGC64900.06.33056971
GTATGTC70800.06.32114931
GTATTCA97950.06.29179141
GTATAAC52700.06.12549971
GGTATCT89850.06.12410551
GTATACA44650.06.0796661
GTATGCT77300.06.0743521
GTCATTA56800.06.0708431
GTATCCA89550.05.9807651
GTATTAA53000.05.95239931
Produced by FastQC (version 0.11.5)