FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17154021
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCAA167500.017.7931846
GCGCAAA170750.017.0750187
TGTCCAG182300.016.7871321
CGCAAAA193100.015.5460728
CAGCGCA195950.015.3572825
TCCAGCG228800.013.1530483
GTCCAGC232050.013.1369432
ACCTAGG43050.013.00952465-69
CCTAGGG42900.012.9543365-69
CCAGCGC249450.012.0353484
GTCGAAC170150.011.9316495
CTAGGGG45200.011.75368170-74
GCAAAAC262450.011.6576029
TCGAACG172400.011.3997026
TCTCGTC178400.010.8037941
CGAACGG193050.010.4786027
CGTCGAA202900.09.8998424
CTTAGGC48600.09.538993140-144
CTCGTCG213100.09.266222
CGCTTAG52000.09.053726140-144
Produced by FastQC (version 0.11.5)