FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17274836
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTGT55600.032.114869
GGTAGTG108200.017.500798
ATCCGAC117000.016.2510151
ACGGTAG125350.015.2787476
CGGTAGT134500.013.8646177
TCCGACG163200.011.9565862
CTAGCTC13450.011.45573445-49
CTAGCCT13500.011.41333820-24
GACGGTA174700.011.3336595
GACTAGC13700.010.61606320-24
ACTAGCC14550.010.58969420-24
GGACTAG14700.010.18765415-19
TCTAGCT17000.09.31762445-49
CGACGGT215800.09.0416884
CAAGTTG95100.07.87377937
TTCTAGC20150.07.57516745-49
TCAAGTT123650.06.6380596
AGTTGAT128050.06.3537199
CACTCTA27701.11595E-86.24013331
AAGTTGA130850.06.1627348
Produced by FastQC (version 0.11.5)