FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13336698
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTGT40950.041.8459789
GGTAGTG67800.025.9113968
ACGGTAG91000.019.5427766
CGGTAGT97800.018.1103537
ATCCGAC109800.016.9885671
GACGGTA147650.012.3859965
GGACTAG10750.011.65386115-19
ACTAGCC10150.011.63346620-24
GACTAGC10500.011.108541520-24
CTAGCTC10250.011.09853245-49
CTAGCCT10750.010.98415720-24
TCCGACG181450.010.396562
CAAGTTG62350.010.39297
TCTAGCT12700.09.52440645-49
CGACGGT217050.08.6578454
AAGTTGA89650.07.9508878
TCAAGTT88400.07.9004196
AGTTGAT101650.06.9414379
TTCTAGC16750.06.70566945-49
TTCAAGT103700.06.3181995
Produced by FastQC (version 0.11.5)