FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17964440
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTAG11650.012.85570995-99
GGAAGCT201850.012.0504334
GCTAGTC12950.011.780852120-124
AGGCTAG12500.011.398974120-124
AGCTAGA13800.011.063560595-99
GGCTAGT13700.010.7156925120-124
GGGAAGC226600.010.5811093
AAGCTGA239150.09.5691086
AGCTGAA228500.09.542757
GCTAGAA16450.09.369678595-99
GCTGAAG259100.08.6657138
TCGGGAA284250.08.5617611
CTAGTCC12300.07.956909120-124
CGGGAAG316100.07.69908432
GAAGCTG342350.07.33617735
CTGAAGC165250.06.96779979
GATCTTA65700.06.137191
CGTAACT70200.06.0482829
CTAGATC7758.54925E-115.7570577140-144
TAACTTG55400.05.4557789
Produced by FastQC (version 0.11.5)