FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16339270
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACATAC87000.012.324595
ATACAAC92250.011.3957921
CAACATA94500.011.2704344
GGCCTAA23750.09.09001175-79
ACATACT121950.08.85145956
ACAACAT141650.08.2809813
TACAACA131150.08.2329352
CATACTC149850.08.1158767
CTCACTA26600.08.06210115-19
ATACTCA144700.07.5592818
TACTCAA165750.07.1637189
CCCTTAA85400.06.48848964
GTACCCT95050.06.06032471
CTACACA38700.06.025416445-49
TACACAC38500.05.86943350-54
ACCCTTA108100.05.4588223
CATTAGG42950.05.3950896100-104
ACACACT42700.05.35952750-54
Produced by FastQC (version 0.11.5)