FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17455701
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAG15100.012.3962114
TACTAAG18900.011.4338412
ACTAAGA19300.010.4505323
ACTAGCT5550.08.561522520-24
CCACTAG5200.08.30707220-24
CTAGCTA4409.094947E-128.18119725-29
CACTAGC6900.06.677761620-24
CTACTGA31701.8189894E-116.5861649
CTAGATC5551.659315E-55.188938140-144
TTACTAA45006.85759E-105.1534511
Produced by FastQC (version 0.11.5)