FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17455701
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA39600.026.3601426
GCTACTG74650.014.4657619
CGCTACT87450.012.5953868
ACGCTAC98500.011.3285767
AGGTACG119000.09.8004653
AAGGTAC133050.08.9824642
GTACGCT127800.08.6749555
CTAAGAG16206.6875873E-77.55461844
GGTACGC178300.06.8236074
CTACTGA36300.06.425608610-14
AAAGGTA198500.06.28280351
ACTAAGA21156.5205113E-66.12689973
TGCTAAG40600.05.8159876135-139
TACTAAG22852.2658462E-55.6713772
CTAGCTA5102.2633682E-55.64666562
TTAACGT48700.05.43987620-24
ACTAGCT6801.4786747E-75.29335820-24
CTAAGGC44600.05.13296135-139
TAACGTT47550.05.026413425-29
Produced by FastQC (version 0.11.5)