FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13662772
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCAG47800.017.552291
TAGACCC32150.017.4635738
GTTAGAC36750.015.2779386
TTAGACC38600.014.1726867
AGACCCC53250.010.9489839
TCTCAGC85000.010.6783312
GCAACAC93900.010.195587
TAGTTAG64800.09.7754134
CTCAGCA111500.09.2318473
CAACACT103850.09.2185938
AGTGAGT28550.07.80186371
GTGAGTA32300.07.58277562
TAAGGGG29950.07.4502919
AGTTAGA92500.06.6924355
AACACTA157750.06.38803159
CAGCAAC174200.06.23959265
TTAGCCC34600.05.8249755120-124
ATGTAGT113600.05.22871351
AGCAACA232250.05.1449346
TAGACTC37950.05.045597135-139
Produced by FastQC (version 0.11.5)