FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15964872
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTGT72350.036.820859
ATCCGAC122850.022.6874031
GGTAGTG124500.021.7444848
ACGGTAG141100.018.9311716
CGGTAGT145750.018.524797
TCCGACG169800.016.1562162
GACGGTA191650.014.4638045
CGACGGT233250.012.2545714
GGACTAG12300.012.05844715-19
CTAGCCT13500.010.98658520-24
CTAGCTC13200.010.799952545-49
TCTAGCT14350.010.5365445-49
GACTAGC14050.010.35152420-24
ACTAGCC15250.09.72582920-24
CAAGTTG96900.08.693467
TTCTAGC16900.08.26505345-49
CCGACGG373300.07.9850143
TCAAGTT119150.07.1304776
AAGTTGA126400.06.9493378
CACTCTA36100.06.7829821
Produced by FastQC (version 0.11.5)