FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14112975
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGATTGGTATGGTGTAGATCTCGGT294050.20835436894063794Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA222250.023.45811
AGAGCGT228400.022.7795858
TCGGAAG252050.020.5570933
GAGCGTC258450.020.4373539
AAGAGCG267650.019.3045987
ATCGGAA285150.018.3527682
GAAGAGC320700.016.2010576
GGAAGAG338700.015.5100365
CGGAAGA379700.013.7597944
TAGATCT80450.012.89874440-44
GTAGATC117450.09.28861635-39
GTAGGGA114350.09.23841615-19
TCATTAA120250.09.2045560-64
AGATCTC118650.09.17048440-44
GTGTAGA123900.08.9212335-39
TGTAGGG122300.08.7084915-19
TAGGGAA123050.08.5267515-19
GTATGGT131500.08.28523730-34
GATTGGT125650.08.21283325-29
TCGTGTA133000.08.14851510-14
Produced by FastQC (version 0.11.5)