FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12808915
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCTCGTAT144750.11300722973023086TruSeq Adapter, Index 16 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC43100.020.3789169
AGTGAGT45100.019.4468921
GTGAGTA49100.017.7442112
ATAACCC73300.012.4737858
GTCTCTT81900.012.0363941
AGCACAT126150.08.960069
GAGTATA122850.08.0298054
GTATAAC123100.07.9539176
TGAGTAT125400.07.8090993
CAGCACA161100.07.41841468
TATAACC136350.07.33938937
GAGCACA345100.07.1973519
CTCTTCA149050.06.90817983
TCAGCAC170050.06.7739757
AGAGCAC361750.06.76657538
AACCCCC31900.06.093472510-14
TCTCTTC167900.06.08962352
TCTTCAG183650.06.0379484
CTAGATC30850.05.835417140-144
TAGATCG127700.05.785535140-144
Produced by FastQC (version 0.11.5)