FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20986836
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAA51200.010.8290224
TACGTTA58850.09.2989433
ACCACTA57950.08.6945799
CTAGACC20450.08.451009120-124
CCACCTA62950.08.34701257
ATACAAC130200.08.1543661
GTTACGT79050.07.87318041
TACAACC143600.07.57196472
ACAACCA137000.07.3582393
CAACCAC137500.07.33151634
AACCACC149750.07.06567145
CACCACT83600.06.97401248
ATCCTAG34150.05.861673115-119
CTAACAC36350.05.78217325-29
CCTAGAC17750.05.760463115-119
CTAGATC11650.05.5621815140-144
CCCTAAT35700.05.4865875125-129
CGTTAAA106150.05.42466455
ACCTACT113100.05.21861469
TTACGTT121650.05.090642
Produced by FastQC (version 0.11.5)