FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16158117
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGTAATCTCGTAT179950.11136817489315123TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA185900.016.4231749
AGAGCAC191750.016.1851968
GATCGGA204950.015.768851
TCGGAAG239400.012.8820033
AAGAGCA252850.012.2171637
ATCGGAA270250.011.6764732
GAAGAGC323250.09.6900676
GGAAGAG336000.09.536675
AGTCACT67300.09.07164725-29
CGGAAGA360300.08.8742094
TCACTGG83550.07.3417430-34
CAGTCAC90050.07.19555225-29
CCAGTCA91300.07.081265425-29
TAATCTC90750.06.822738640-44
GTCACTG95650.06.563520425-29
GTAATCT96000.06.55465935-39
CTCGTAT95950.06.34793340-44
GTGGTAA106550.05.905652535-39
TCTCGTA108500.05.75966140-44
CTAGATC11150.05.682708140-144
Produced by FastQC (version 0.11.5)