FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16158117
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGATTCGCACCGTGTAGATCTCGGT183480.11355283539536197Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA207500.014.731481
AGAGCGT242150.012.2480238
TCGGAAG248700.012.0990443
ATCGGAA274000.011.2733442
GAGCGTC279700.010.6551899
AAGAGCG290050.010.3990687
GAAGAGC326850.09.4484246
GGAAGAG341000.08.9507495
TAGATCT71350.08.55607940-44
CGGAAGA371800.08.2091914
TCATTAA79950.07.83416660-64
CATTAAA100450.06.57937660-64
TAGGGAA102550.06.303930815-19
ATCATTA101400.06.10593960-64
GTAGATC105650.06.09180535-39
GTAGGGA105250.06.07381615-19
GTGTAGA110150.05.97364935-39
AGATTCG109400.05.685867325-29
TGTAGGG114450.05.64847615-19
TGTAGAT116750.05.537292535-39
Produced by FastQC (version 0.11.5)