FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15038579
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACTC51150.035.897888
ACGTTAA60650.030.9905134
CCACTCA69950.025.940599
CGTTAAA80550.025.2091275
GTTACGT97350.019.3914891
TACGTTA100950.018.9890633
AAGCCAC107050.017.4888386
AGCCACT138750.013.7526747
AACACTA71900.013.7203747
CAAACAC80600.012.5074065
ACAAAGC154050.011.9758543
ACACTAT88250.011.5047898
TTACGTT172250.011.5036382
AAACACT93150.010.8222976
GTTAAAT204000.010.3421716
CCAAACA121000.08.748854
TAGCCCT80100.08.6357572
GTAGCCC78400.08.3029621
TACAAAG244050.08.2373432
GTCCCCT39150.07.9812193100-104
Produced by FastQC (version 0.11.5)